Annexin V-iFluor 488 + PI Apoptosis Detection Reagent
Croyez fluorescent dye (iFluor 488) conjugated Annexin V is highly purified product. During early apoptosis, cells will translocate membrane phosphatidylserine (PS) from the inner face of the membrane to the cell surface. Propidium iodide (PI) is a common fluorescent dye to detect DNA. It can be used in flow cytometry to evaluate the cell cycle and cell viability during apoptosis. The
product can be used in one-step staining procedure without wash step within 20 minutes.
product can be used in one-step staining procedure without wash step within 20 minutes.
Component:
Storage:
Store at 4°C in the dark.
All reagents are stable for one year under proper storage conditions.
Manual:
1. Collect 1-5x105 cells in the flow tube by centrifugation.
2. Wash cells in 2 mL cold phosphate-buffered saline (PBS) and collect by centrifugation.
3. Re-suspend cell in 500 μL of 1X Binding Buffer.
4. Add 5 μL of Annexin V-iFluor 488 and 5 μL of PI, gently mix the cells and incubate for 15-20 minutes at RT in the dark.
5. After incubation, the samples should be kept on ice and perform flow cytometry using filters appropriated for fluorescein
(FITC, corresponding to Annexin V-iFluor 488).
Shipping Conditions:
Blue ice
| C08006-K01 | C08006-K02 | C08006-K03 | |
| Annexin V-iFluor 488 |
1 x 0.125 mL | 1 x 0.25 mL | 1 x 0.5 mL |
| PI | 1 x 0.125 mL | 1 x 0.25 mL | 1 x 0.5 mL |
| 10X Binding Buffer | 1 x 2 mL | 2 x 2 mL | 3 x 2 mL |
Storage:
Store at 4°C in the dark.
All reagents are stable for one year under proper storage conditions.
Manual:
1. Collect 1-5x105 cells in the flow tube by centrifugation.
2. Wash cells in 2 mL cold phosphate-buffered saline (PBS) and collect by centrifugation.
3. Re-suspend cell in 500 μL of 1X Binding Buffer.
4. Add 5 μL of Annexin V-iFluor 488 and 5 μL of PI, gently mix the cells and incubate for 15-20 minutes at RT in the dark.
5. After incubation, the samples should be kept on ice and perform flow cytometry using filters appropriated for fluorescein
(FITC, corresponding to Annexin V-iFluor 488).
Shipping Conditions:
Blue ice
1.
Li R, Inbaraj BS, Chen BH. Quantification of Xanthone and Anthocyanin in Mangosteen Peel by UPLC-MS/MS and Preparation of Nanoemulsions for Studying Their Inhibition Effects on Liver Cancer Cells. Int J Mol Sci. 2023 Feb 15;24(4):3934.
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